Journal: PLoS Neglected Tropical Diseases

Article Title: Intranasal Vaccination with Leishmanial Antigens Protects Golden Hamsters ( Mesocricetus auratus ) Against Leishmania (Viannia) braziliensis Infection

doi: 10.1371/journal.pntd.0003439

Figure Lengend Snippet: Golden hamsters (5–6 per group) were immunised with IN LaAg (•), IM LaAg (▴) or PBS (□). Two weeks after the last immunisation, hamsters were challenged with L. braziliensis . A) IgG and IgG2 serum levels were determined 114 days post-infection by ELISA. B) Correlation between lesion thickness and anti-leishmanial IgG (r = 0.78, p<0.0001) or IgG2 (r = 0.62, p<0.0001) serum levels. Each point represents one animal. The horizontal bars represent the median values, * p≤0.05. Data are from two independent experiments.

Article Snippet: Plasma samples were diluted 1∶5000 for IgG and 1∶200 for IgG2 and detected by horseradish peroxidase-labelled goat anti-hamster IgG (Santa Cruz Biotechnology, Santa Cruz, California, USA) and biotin–conjugated mouse anti-Syrian hamster IgG2 (Becton Dickinson, New Jersey, USA).

Techniques: Infection, Enzyme-linked Immunosorbent Assay

Journal:

Article Title: Activation of T-cell receptor-?? + cells in the intestinal epithelia of KN6 transgenic mice

doi: 10.1046/j.1365-2567.2000.00076.x

Figure Lengend Snippet: Proliferative response of splenic Tg TCR-γδ+ cells and Tg γδ-IEL in KN6-Tg mice of BALB/c background. (a) Proliferation in response to irradiated H-2d (○, ▵) or H-2b (•, ▴) splenocytes. (b) Proliferation stimulated by immobilized hamster IgG (○, ▵) or anti-Vγ4 mAb (•, ▴). Circles represent the response of splenic Tg TCR-γδ+ cells, and triangles indicate the response of Tg γδ-IEL.

Article Snippet: Biotinylated antibodies were detected by phycoerythrin-conjugated streptavidin (Caltag Laboratories, Burlingame, CA), and anti-CD103 antibody was detected by biotinylated anti-hamster immunoglobulin G (IgG; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then by phycoerythrin-conjugated streptavidin.

Techniques: Irradiation

Journal:

Article Title: Activation of T-cell receptor-?? + cells in the intestinal epithelia of KN6 transgenic mice

doi: 10.1046/j.1365-2567.2000.00076.x

Figure Lengend Snippet: IFNγ production by splenic Tg TCR-γδ+ cells and Tg γδ-IEL in KN6-Tg mice of BALB/c background. Tg TCR-γδ+ cells purified from spleen (▵, ▴) or intestinal epithelia (○, •) were cultured in the presence of immobilized hamster IgG (open symbols) or anti-TCR-δ mAb (closed symbols), and concentration of IFN-γ in the supernatants was measured.

Article Snippet: Biotinylated antibodies were detected by phycoerythrin-conjugated streptavidin (Caltag Laboratories, Burlingame, CA), and anti-CD103 antibody was detected by biotinylated anti-hamster immunoglobulin G (IgG; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) and then by phycoerythrin-conjugated streptavidin.

Techniques: Purification, Cell Culture, Concentration Assay

Journal: eLife

Article Title: Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms

doi: 10.7554/elife.97532

Figure Lengend Snippet: Figure 2. Immunogenicity of the vaccine candidate in hamsters. (A) Hamsters were inoculated with 1 × 103 or 1 × 104 plaque-forming unit (PFU) of BK2102 intranasally, and the serum was collected 4 weeks after inoculation. Spike-specific IgG in the sera of BK2102-inoculated hamsters and mock- treated hamsters was detected by ELISA. Symbols depict data of individual hamsters (n=10), and bars correspond to the median value. The limit of dilution is indicated in the x-axis. (B) Neutralizing antibodies in the sera were induced in BK2102-inoculated hamsters. Neutralizing antibodies in the

Article Snippet: Biotinylated anti- Syrian hamster IgG1 Ab (Southern Biotech, Cat# 1940- 08, 1/100 dilution), IgG2/3 subclass Ab (Southern Biotech, Cat# 1935- 08, 1/200,000 dilution), and IgA Ab (Brookwood Biomedical, Cat# 3003a, 1/100 dilution) were used and detected with HRPlabeled streptavidin (Abcam, Cat# ab7403, 1/10,000 dilution).

Techniques: Immunopeptidomics, Enzyme-linked Immunosorbent Assay

Journal: Cell reports

Article Title: Blood flow patterns switch VEGFR2 activity through differential S-nitrosylation and S-oxidation

doi: 10.1016/j.celrep.2023.113361

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cy3-conjugated goat anti-hamster , Jackson ImmunoResearch , Cat# 107-166-142; RRID:AB_2337465.

Techniques: Virus, Recombinant, Biotin Switch Assay, Mutagenesis, Control, Sequencing, Software

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

Figure Lengend Snippet: Characterization of purified AT1 cells (A) Immunofluorescence confocal microscope images of AT1 cells cultured for 5 days. Aquaporin5 (AQP5) is a marker of AT1 cells. Scale bar: 50 μm. (B) Quantification of AQP5-positive cells. (C) Dot plot of purified cells cultured for 5 days stained with anti-RAGE, AT1 cells were gated as the black polygon and singlets were gated as the diagonal. FSC, forward scatter; SSC, side scatter; FSC-H, forward scatter height; FSC-A, forward scatter area. (D) Dot plot of gated single AT1 cells with AF488 fluorescence intensity as X axis. (E) Dot plot of purified cells cultured for 5 days stained with anti-PDPN, AT1 cells were gated as the black polygon and singlets were gated as the diagonal. (F) Dot plot of gated single AT1 cells with CY3 fluorescence intensity as X axis. (G) Interleukin-6 concentration in the supernatants of AT1 cells incubated for 24 h with or without anti-RAGE antibody. (H) Viability of AT1 cells at different time points as day 5, 8, 12, and 14 (D5, D8, D12, D14). Data represent mean ± SD. ∗∗∗∗, p < 0.0001.

Article Snippet: Biotinylated Syrian hamster anti-mouse podoplanin (PDPN) monoclonal antibody (eBio8.1.1 (8.1.1)) (1:50 dilution) , Thermo Fisher Scientific , Cat# 14-5381-82; RRID: AB_1210505.

Techniques: Purification, Immunofluorescence, Microscopy, Cell Culture, Marker, Staining, Fluorescence, Concentration Assay, Incubation

Journal: STAR Protocols

Article Title: Protocol for preparation of primary alveolar epithelial type I cells from mouse lungs

doi: 10.1016/j.xpro.2024.103484

Figure Lengend Snippet:

Article Snippet: Biotinylated Syrian hamster anti-mouse podoplanin (PDPN) monoclonal antibody (eBio8.1.1 (8.1.1)) (1:50 dilution) , Thermo Fisher Scientific , Cat# 14-5381-82; RRID: AB_1210505.

Techniques: Recombinant, Modification, Electron Microscopy, Software, Sterility